Dr. Marshall is an analytical biochemist. Our main interest is in proteins, peptides and enzymes located at or near the cell surface and their involvement in extra-cellular processes. The research program in the protein biochemistry laboratory at Ryerson University includes isolation, primary sequence identification and/or quantification of polypeptides and their activities by biochemical, biophysical, immunological or cytological methods. Our goal is to discover the proteins from blood, white blood cells, and their receptors on the cell surface that are of central importance in the disease diagnosis and therapy. Our analytical approach is to combine biochemical, enzymological, mass spectrometric (MS), cytological, laser confocal. immunological and statistical methods into a comprehensive analytical system.

1. Jankowski A, Zhu P, Marshall JG (2008) Capture of an activated receptor complex from the surface of live cells by affinity receptor chromatography. Analytical Biochemistry 380: 235-248.

2. Monika Tucholska, Salvatore Scozzaro, Declan Williams, Suzanne Ackloo, Chris Lock, K.W. Michael Siu, Kenneth R. Evans and John G. Marshall (2007) Endogenous Peptides from Biophysical and Biochemical Fractionation of Serum Analyzed by MALDI and ESI Qq-TOF. Analytical Biochemistry. 15;370(2):228-45

3. Peihong Zhu, Peter Bowden, Voitek Pendrak, Herbert Thiele, Du Zhang, Michael Siu, Eleftherios P. Diamandis and John Marshall (2007) Comparison of Protein Expression Lists From Mass Spectrometry of Human Blood Fluids Using Exact Peptide Sequences Versus BLAST Clinical Proteomics 2 1-20.

4. Declan Williams, Peihong Zhu, Peter Bowden, Catherine Stacey, Mike McDonell, Paul Kowalski, Jane Marie Kowalski, Ken Evans, Eleftherios P Diamandis, K.W. Michael Siu and John Marshall (2007) Comparison of Methods to Examine the Endogenous Peptides of Fetal Calf Serum. Clinical Proteomics. January 2007 Vol 2(1) 67-90.

5. Corbett, E, Mason D, Marshall J, Collette, Grinstein S. (2006) Signaling-dependent immobilization of acylated proteins in the inner monolayer of the plasma membrane. J Cell Biol. Jul 17;174(2):255-65.

6. John Marshall, Andy Jankowski, Inga Kireeva, Lisa Barker, Mila Dumbrovsky, Weimin Zhu,Kellie Jacks, Leslee Ingratta, Jenny Bruin, Erika Kristensen and Rulin Zhang, Eric Stanton, MiyokoTakahashi and George Jackowski (2004) Human Serum Proteins Pre-Separated by Electrophoresis or Chromatography Followed by Tandem Mass Spectrometry. Journal of Proteome Research 3(3) 364-382.

7. Rulin Zhang, Lisa Barker, Deborah Pinchev, John Marshall, Michele Rasamoelisolo, Chris Smith, Peter Kupchak, Inga Kireeva, Leslee Ingrata and George Jackowski. (2004) Mining Biomarkers in Human Sera Using Proteomic Tools. Proteomics (1):244-256.

8. John Marshall, Peter Kupchak, Weimin Zhu, Jason Yantha, Tammy Vrees, Shirley Furesz, Kelly Jacks, Chris Smith, Inga Kireeva, Rulin Zhang, Miyoko Takahashi, Eric Stanton, and George Jackowski. (2003) Processing of Serum Proteins Underlies the Mass Spectral Fingerprinting of Myocardial Infarction. Journal of Proteome Research 2(4):361-372.

9. Marshall JG, Booth JW, Sambolic V, Mak T, Balla T, Schreiber AD, Meyer T, Grinstein S. (2001). Restricted accumulation of phosphatidyl inositol 3-kinase products in a plasmelemmal subdomain during Fcg receptor-mediated phagocytosis. Journal of Cell Biology 153:1369-1380.

10. Marshall J., Krump K., Lindsay T., Downey G., Ford D.A., Zhu P-H, Walker P., Rubin B. (2000). Involvement of cytosolic phospholipase A2 and secretory phospholipase A2 in arachidonic acid release from human neutrophils. Journal of Immunology 164(4):2084-91.