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Research Technician - Protein expression and purification

Department: Chemistry and Biology
Position supervisor: Dr Michael Olson
Contract length: 2 years
Hours of work per week: 36.25
Position type: TERM Vacancy
Rate of pay: To be determined

About Ryerson

At the intersection of mind and action, Ryerson is on a transformative path to become Canada’s leading comprehensive innovation university. Integral to this path is the placement of equity, diversity and inclusion as fundamental to our institutional culture. Our current academic plan outlines each as core values and we work to embed them in all that we do.

Ryerson University welcomes those who have demonstrated a commitment to upholding the values of equity, diversity, and inclusion and will assist us to expand our capacity for diversity in the broadest sense. In addition, to correct the conditions of disadvantage in employment in Canada, we encourage applications from members of groups that have been historically disadvantaged and marginalized, including First Nations, Metis and Inuit peoples, Indigenous peoples of North America, racialized persons, persons with disabilities, and those who identify as women and/or 2SLGBTQ+. Please note that all qualified candidates are encouraged to apply; however, applications from Canadians and permanent residents will be given priority.

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About the program/department/team

Professor Michael Olson is Canada Research Chair in Molecular Cell Biology and Professor in the Ryerson University Department of Chemistry and Biology. The Olson laboratory is located in newly built biomedical research facilities at the MaRS Discovery District (marsdd.com), external link in downtown Toronto (at Queens Park subway station), within one of the highest densities of biomedical research activity in North America.

The Olson lab works on Rho GTPase regulation of the actin cytoskeleton, and the role of associated signalling pathways in cancer. Recent examples of the labs work include: (Cancer Res. 2018;78:2096, external link; ACS Chem Biol. 2016;11:3300, external link; Curr Biol. 2015 Jun 1;25(11):1520, external link).

The opportunity

The incumbent will work under the general direction of the Principal Investigator (PI), and will support the PI, students, post-doctoral fellows (PDFs) in the laboratory. The research responsibilities will include: design and production of DNA constructs for protein expression in various systems including bacteria and mammalian cells; expression and purification of proteins from systems including bacteria and mammalian cells, in vitro biochemical assays with recombinant proteins, evaluation and implementation of conditions for protein crystal formation, growth of antibody-producing hybridoma cell lines for monoclonal antibody production, purification of monoclonal antibodies from hybridoma conditioned medium.  In addition to these laboratory responsibilities, the incumbent will also be responsible for training, troubleshooting and fulfilling all maintenance requirements for microscopy equipment, inclusive of equipment and space, funded through awards and matching funds from the Canada Foundation for Innovation.

Qualifications

The ideal candidate holds a Bachelor’s or Master’s degree in a relevant scientific discipline, such as biochemistry or cell biology. Interested individuals must demonstrate their expertise in protein expression and purification. This position is not tied to a particular research project, but will support the research projects undertaken by team members. In addition, the incumbent will be directly involved in the operation and maintenance of newly-acquired infrastructure. Expertise in image acquisition software is highly desired, as is familiarity with common image analysis software programs. Experience with safe lab operations, including all safety procedures, restocking lab supplies and reagents, troubleshooting and the ability to provide effective training and orientation to students and other researchers is essential. The incumbent should be highly computer literate, have excellent interpersonal and communication skills, and be able to work in a collaborative and multidisciplinary research group.

How to apply

Please email your application to Michael Olson at application@olsonlab.ca. Applications should be submitted in screen readable PDF or word formats. In the subject line please indicate: “Application”

Ryerson’s commitment to equity, diversity and inclusion

We encourage all First Nations, Metis and Inuit peoples or Indigenous peoples of North America, to self-identify in their applications and also reach out to Tracey King, Aboriginal HR Consultant  for support during the selection process.

Please watch a message from Ryerson's Vice President, Equity and Community Inclusion for more information on our commitment to equity.