Research Technician - Protein expression and purification
Department: Chemistry and Biology
Position supervisor: Dr Michael Olson
Contract length: 2 years
Hours of work per week: 36.25
Position type: TERM Vacancy
Rate of pay: To be determined
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About the program/department/team
Professor Michael Olson is Canada Research Chair in Molecular Cell Biology and Professor in the Ryerson University Department of Chemistry and Biology. The Olson laboratory is located in newly built biomedical research facilities at the MaRS Discovery District (marsdd.com), external link in downtown Toronto (at Queens Park subway station), within one of the highest densities of biomedical research activity in North America.
The Olson lab works on Rho GTPase regulation of the actin cytoskeleton, and the role of associated signalling pathways in cancer. Recent examples of the labs work include: (Cancer Res. 2018;78:2096, external link; ACS Chem Biol. 2016;11:3300, external link; Curr Biol. 2015 Jun 1;25(11):1520, external link).
The incumbent will work under the general direction of the Principal Investigator (PI), and will support the PI, students, post-doctoral fellows (PDFs) in the laboratory. The research responsibilities will include: design and production of DNA constructs for protein expression in various systems including bacteria and mammalian cells; expression and purification of proteins from systems including bacteria and mammalian cells, in vitro biochemical assays with recombinant proteins, evaluation and implementation of conditions for protein crystal formation, growth of antibody-producing hybridoma cell lines for monoclonal antibody production, purification of monoclonal antibodies from hybridoma conditioned medium. In addition to these laboratory responsibilities, the incumbent will also be responsible for training, troubleshooting and fulfilling all maintenance requirements for microscopy equipment, inclusive of equipment and space, funded through awards and matching funds from the Canada Foundation for Innovation.
The ideal candidate holds a Bachelor’s or Master’s degree in a relevant scientific discipline, such as biochemistry or cell biology. Interested individuals must demonstrate their expertise in protein expression and purification. This position is not tied to a particular research project, but will support the research projects undertaken by team members. In addition, the incumbent will be directly involved in the operation and maintenance of newly-acquired infrastructure. Expertise in image acquisition software is highly desired, as is familiarity with common image analysis software programs. Experience with safe lab operations, including all safety procedures, restocking lab supplies and reagents, troubleshooting and the ability to provide effective training and orientation to students and other researchers is essential. The incumbent should be highly computer literate, have excellent interpersonal and communication skills, and be able to work in a collaborative and multidisciplinary research group.
How to apply
Please email your application to Michael Olson at email@example.com. Applications should be submitted in screen readable PDF or word formats. In the subject line please indicate: “Application”
Ryerson’s commitment to equity, diversity and inclusion
We encourage all First Nations, Metis and Inuit peoples or Indigenous peoples of North America, to self-identify in their applications and also reach out to Tracey King, Aboriginal HR Consultant for support during the selection process.
Please watch a message from Ryerson's Vice President, Equity and Community Inclusion for more information on our commitment to equity.